Your Independent Sequencing Guide
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Related Technologies > BioNano Genomics
The Irys System’s ($295k) main claim to fame is its ability to analyze very
long (at least up to 1Mb), unamplified stretches of DNA at a rate of several Gb
per hour. It does this by linearizing DNA molecules through massively parallel
nanochannels. However, rather the sequencing individual bases, the multi-color
imaging instrument detects sites that have been fluorescently labeled via a
site-specific nicking endocuclease/polymerase repair reaction. These labeled
sites, which tend to be several kb apart, generate positional information
across the entire length of the DNA molecule. This macro-level analysis could
be used on its own (e.g, structural variation analysis) or in conjunction with
NGS data (e.g., sequence assembly).