Your Independent Sequencing Guide
Design your 454 next-gen sequencing experiment with BlueSEQ's online tools.
Sequencing Platforms > 454 (Roche)
The GS FLX System is 454′s flagship sequencing system. The current specifications, when operated with the Titanium series reagents, offer up to 1M reads with a Q20 read length of 400 bases in a 10 hour run. It requires the use of emulsion PCR (emPCR) and has a higher cost per base than the NGS market leaders. It is typically used in situations where long reads are at a premium and the number of reads/runs aren’t critical.
The GS Junior is a less powerful version of the GS FLX system, offering about one tenth the output of the FLX but at one fourth the instrument cost: ~100k reads with an average Q20 read length of 400 bases in a 10 hour run. Its main attractions are the small footprint (about the size of a laser printer) and the less expensive runs (albeit at a higher cost per base) and it is geared for those researchers who have modest sequencing needs.
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